The data originally presented from the study was interpreted beyond the sensitivity for both the GC-MS and LC-MS-MS. The purpose for this commentary is to reinterpret the data from the original study by providing the sensitivity and more detailed methodology for both the GC-MS and LC-MS-MS used.
GC-MS- Sensitivity when screening for fentanyl for the BioSPME method, the following calibrator solutions were prepared by diluting a 1 mg/mL methanolic fentanyl stock standard: 5, 10, 15, 25, 50, 75, and 100 μg/mL. A calibration curve was generated by analyzing calibrator solutions in triplicate. Figures of merit data, equation for regression (R2), Limit of Detection (LOD), and Limit of Quantitation (LOQ) were obtained from the calibration curve.
LC-MS-MS- A calibration curve was generated by analyzing extracted samples in triplicate over two days using the C18 BioSPME fiber. Figures of merit data, coefficient of determination (R2), Limit of Detection (LOD), and Limit of Quantitation (LOQ) were obtained from the calibration curve. The fentanyl extraction calibrator solutions produced a Linear Dynamic Range (LDR) from 1-500 ng/mL with an R2 value of 0.9927 and 0.9994 for the 188 and 105 ions, respectively.
